0000000001304785

AUTHOR

David N. Sheppard

showing 3 related works from this author

Efficient, non-toxic anion transport by synthetic carriers in cells and epithelia.

2016

Transmembrane anion transporters (anionophores) have potential for new modes of biological activity, including therapeutic applications. In particular they might replace the activity of defective anion channels in conditions such as cystic fibrosis. However, data on the biological effects of anionophores are scarce, and it remains uncertain whether such molecules are fundamentally toxic. Here, we report a biological study of an extensive series of powerful anion carriers. Fifteen anionophores were assayed in single cells by monitoring anion transport in real time through fluorescence emission from halide-sensitive yellow fluorescent protein. A bis-(p-nitrophenyl)ureidodecalin shows especial…

Yellow fluorescent proteinpotencyGeneral Chemical Engineeringsynthetic anion carriersCystic Fibrosis Transmembrane Conductance Regulator01 natural sciencesMadin Darby Canine Kidney CellsCell membranedeliverabilityta116Drug CarriersbiologyMolecular StructureChemistryBiological activitypersistenceCystic fibrosis transmembrane conductance regulatorTransmembrane proteinanionophoresmedicine.anatomical_structureBiochemistryPhosphatidylcholinesSteroidsChlorineAnionsCell SurvivalNaphthalenesta3111010402 general chemistryDogsBacterial ProteinsCyclohexanesmedicineAnimalsHumansIon transporterCell ProliferationIon Transport010405 organic chemistryCell MembranetoxicityTransporterEpithelial CellsHydrogen BondingGeneral ChemistryRats Inbred F3440104 chemical sciencesElectrophysiological PhenomenaLuminescent ProteinsMicroscopy FluorescenceCell cultureDrug Designbiology.proteinHeLa CellsNature chemistry
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A NEW MODEL OF CF DISEASE: NO F508, NO KINASE?

2008

Pulmonary and Respiratory Medicine0303 health sciencesKinasebusiness.industryDiseasemedicine.diseaseCystic fibrosis03 medical and health sciences0302 clinical medicine030228 respiratory systemPediatrics Perinatology and Child HealthmedicineCancer researchPediatrics Perinatology and Child Healthbusinessskin and connective tissue diseases030304 developmental biologyJournal of Cystic Fibrosis
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CCDC 1420195: Experimental Crystal Structure Determination

2015

Related Article: Hongyu Li, Hennie Valkenier, Luke W. Judd, Peter R. Brotherhood, Sabir Hussain, James A. Cooper, Ondřej Jurček, Hazel A. Sparkes, David N. Sheppard, Anthony P. Davis|2016|Nature Chemistry|8|24|doi:10.1038/nchem.2384

Space GroupCrystallographyCrystal SystemCrystal StructureCell ParametersNNNN-tetramethylaminium chloride ethyl 27-bis(((4-nitrophenyl)carbamoyl)amino)octahydronaphthalene-4a(2H)-carboxylateExperimental 3D Coordinates
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