Supplementary Material for: Validation of Fenoterol to Study ��2-Adrenoceptor Function in the Rat Urinary Bladder

2021

Fenoterol is a ��2-adrenoceptor (AR)-selective agonist that is commonly used to investigate relaxation responses mediated by ��2-AR in smooth muscle preparations. Some data have questioned this because fenoterol had low potency in the rat urinary bladder when a muscarinic agonist was used as a pre-contraction agent and because some investigators proposed that fenoterol may act in part via ��3-AR. We designed the present study to investigate whether fenoterol is a proper pharmacological tool to study ��2-AR-mediated relaxation responses in the rat urinary bladder. Firstly, we have compared the effect of pre-contraction agents on fenoterol potency and found that fenoterol potency was about 1.…