Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results A dysbiotic endomet…

Prospective, randomized study of the endometrial receptivity analysis (ERA) test in the infertility work-up to guide personalized embryo transfer ver…

2016

A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF.

2020

Abstract Research question Does clinical performance of personalized embryo transfer (PET) guided by endometrial receptivity analysis (ERA) differ from frozen embryo transfer (FET) or fresh embryo transfer in infertile patients undergoing IVF? Design Multicentre, open-label randomized controlled trial; 458 patients aged 37 years or younger undergoing IVF with blastocyst transfer at first appointment were randomized to PET guided by ERA, FET or fresh embryo transfer in 16 reproductive clinics. Results Clinical outcomes by intention-to-treat analysis were comparable, but cumulative pregnancy rate was significantly higher in the PET (93.6%) compared with FET (79.7%) (P = 0.0005) and fresh embr…

In vitro fertilization with personalized blastocyst transfer versus frozen or fresh blastocyst transfer: a multicenter, randomized clinical trial

2019

Additional file 7 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 6 : Supplementary Table 3. Sequencing reads obtained from endometrial fluid and endometrial biopsy samples.

Additional file 8 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 7 : Supplementary Table 4. Bacteria detected in endometrial fluid and endometrial biopsy samples after filtering for low abundance taxa and potential contaminants.

Additional file 5 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 4 : Supplementary Table 1. Sociodemographic and clinical characteristics of study participants.

Additional file 6 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 5 : Supplementary Table 2. Clinical variables in patients with different reproductive outcomes.

Additional file 4 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 3 : Supplementary Figure 3. Confidence intervals and reference ranges of bacterial taxa detected in patients with live birth. The endometrial microbiome from patients with a live birth was analysed in (A) endometrial fluid and (B) endometrial biopsy samples to determine the reference ranges for each evaluated taxon. The confidence interval displays the relative abundance for all assessed patients, revealing the healthy ranges of abundance for the taxa in the tested panel. The healthy distribution was used to define the 95% confidence interval (red line) and taxa abundance in patients with poor reproductive outcomes���no pregnancy, biochemical pregnancy, and clinical miscarri…

Additional file 3 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 2 : Supplementary Figure 2. Taxa enriched in patients with different reproductive outcomes.

Additional file 2 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2022

Additional file 1 : Supplementary Figure 1. Taxa found in endometrial fluid (A) and endometrial biopsy (B) samples.