0000000001309284
AUTHOR
Michel Chalot
Poplar biomass production at phytomanagement sites is significantly enhanced by mycorrhizal inoculation
Abstract Afforestation of trace element (TE)-contaminated soil, notably with fast growing trees producing large biomass has been demonstrated to be an attractive option for their bioremediation. Mycorrhizal fungi can form symbiotic associations with plants, contributing to TE tolerance and participating actively in bioremediation processes. We studied the effects of mycorrhizal inoculation on the growth of two poplar clones (Skado and I214), to large-scale. Two TE-contaminated sites of ca . 1 ha (Pierrelaye and Fresnes-sur-Escaut, France) were planted with 2200 trees, and were either inoculated with a mixed commercial inoculum or not-inoculated and allometric parameters were determined afte…
N transport in the Hebeloma cylindrosporum – Pinus pinaster ectomycorrhizal association
International audience; Studies of nitrogen metabolism in ectomycorrhizas have demonstrated that the fungal symbiont plays a fully integrated role in plant root metabolism and participates actively in the assimilation and transfer of newly absorbed nitrogen compounds by transferring amino acids. In symbiotic associations, such as mycorrhizas, location of the symbiosis ensures that mycelial growth of the fungus into soil provides access to nutrients of the soil solution. Changes in the distribution and activity of membrane-bound transport proteins in response to symbiotic interactions need to be investigated. In recent years several genes from H. cylindrosporum and P. pinaster, putatively in…
Early screening of new accumulating versus non-accumulating tree species for the phytomanagement of marginal lands
International audience; The use of fast-growing trees producing a high quantity of biomass can bring significant practical and economic benefits to the reclamation of marginal lands. The present study aims to identify new shrub/tree species to offer a wider range of shrubs/trees useful for phytomanagement practices. We implemented three experimental sites in France of 1 ha each (Thann, Carrières-sous-Poissy and Leforest) contaminated by different potentially toxic elements (PTE) with a total of 38 different tree species. After two years of growth, the element concentrations in stem and leaf biomasses, tree survival rate and growth of plants were assessed. Although the three sites had elevat…
Poplar rotation coppice at a trace element-contaminated phytomanagement site: A 10-year study revealing biomass production, element export and impact on extractable elements
Abstract Growing lignocellulosic crops on marginal lands could compose a substantial proportion of future energy resources. The potential of poplar was explored, by devising a field trial of two hectares in 2007 in a metal-contaminated site to quantify the genotypic variation in the growth traits of 14 poplar genotypes grown in short-rotation coppice and to assess element transfer and export by individual genotypes. Our data led us to conclusions about the genotypic variations in poplar growth on a moderately contaminated site, with the Vesten genotype being the most productive. This genotype also accumulated the least amounts of trace elements, whereas the Trichobel genotype accumulated up…
Environmental metabarcoding reveals contrasting microbial communities at two poplar phytomanagement sites
The aim of the present study is to deepen the current understanding of the microbial communities at two poplar phytomanagement sites to reveal the environmental factors that drive the abundance, diversity and composition of microbial communities. A soil analysis revealed that the two soils displayed contrasting physico-chemical characteristics, with significant lower pH and higher Cd, Zn and Mn CaCl2-extractable fractions at Leforest site, compared with Pierrelaye site. The fungal and bacterial community profiles in the poplar roots and soils were assessed through Illumina MiSeq sequencing. Diversity indices and β-diversity measures illustrated that the root microbial communities were well …
Phytoscreening as an efficient tool to delineate chlorinated solvent sources at a chlor-alkali facility
Chlorinated ethenes (CE) are among the most common volatile organic compounds (VOC) that contaminate groundwater, currently representing a major source of pollution worldwide. Phytoscreening has been developed and employed through different applications at numerous sites, where it was generally useful for detection of subsurface chlorinated solvents. We aimed at delineating subsurface CE contamination at a chlor-alkali facility using tree core data that we compared with soil data. For this investigation a total of 170 trees from experimental zones was sampled and analyzed for perchloroethene (PCE) and trichloroethene (TCE) concentrations, measured by solid phase microextraction gas chromato…
Pretreatment of trace element-enriched biomasses grown on phytomanaged soils for bioethanol production
Abstract Phytotechnologies are a set of sustainable, ecological options notably for alleviating pollutant linkages induced by contaminated soils. However, concerns exist regarding the processing of trace elements (TE)-enriched biomass and the fate of TEs in the end-products. The fractionation of phytoremediation TE-enriched non-woody lignocellulosic (tobacco) and woody (birch, willow) phytoremediation-borne biomasses was studied using ethanol organosolv, soda and dilute acid pretreatments. TE distribution in the process fractions (pulp, liquid effluents and lignin) was further examined. In dilute acid conditions, a wood pretreatment performed at 170 °C in the presence of 2% w/w of sulfuric …
Pioneer trees of Betula pendula at a red gypsum landfill harbour specific structure and composition of root-associated microbial communities.
The study of root-associated microbial communities is important to understand the natural processes involved in plant recolonisation at degraded areas. Root associated bacterial and fungal communities of woody species colonising a red gypsum landfill (a metal-enriched environment) were characterised through metabarcoding. Among trees naturally growing on the landfill, Betula pendula is the only tree species in the centre of the area, whereas companion tree species such as Populus nigra, P. tremula and Salix purpurea were present on the edges. The bacterial community was dominated by Proteobacteria (38%), Actinobacteria (35%) and Bacteroidetes (20%) and the most abundant bacterial OTU belong…
Diversity and complexity of microbial communities from a chlor-alkali tailings dump
Abstract Revegetation of the tailings dumps produced by various industrial activities is necessary to prevent dust storms and erosion and represents a great challenge for ecological restoration. Little is known about the microbial colonisation and community structure of revegetated tailings following site exploitation. Here, we report the sequencing of 16S rRNA and internal transcribed spacer (ITS) fungal RNA gene amplicons from chlor-alkali residue and from an adjacent undisturbed soil to define the composition and assembly of the rhizosphere microbial communities. After quality filtering, a total of 72,373 and 89,929 bacterial sequences and 122,618 and 111,209 fungal sequences remained fo…
Interactions between dark septate endophytes, ectomycorrhizal fungi and root pathogens in vitro
ABSTRACT Dark septate endophytes (DSEs) are widely distributed worldwide and can promote plant growth. Therefore, they are considered potentially important plant allies, especially in stressful environments. Previous studies have reported that DSEs cohabit roots with other microorganisms such as ectomycorrhizal (ECM), endophytic and pathogenic fungi/oomycetes. However, interactions between different DSE species have not yet been reported, and studies on the interactions between DSEs and other fungi are scarce. Using a simple and reproducible pairwise growth assay in vitro, we studied the synergistic/antagonistic interactions between eight DSEs, two ECM fungi and three root pathogens. Most o…
Identification of new hardy ferns that preferentially accumulate light rare earth elements: a conserved trait within fern species
Environmental contextRare earth elements (REEs) are strategic metals and emerging contaminants for which plant-based remediation measures are needed. We screened a collection of hardy ferns and identified new accumulator species that preferentially transferred light REEs to their fronds. This study is an important step towards understanding the mechanisms of REE accumulation in plants. AbstractRare earth elements (REEs) include the lanthanides plus yttrium and scandium, and can be split according to their atomic mass into light (LREEs) and heavy REEs (HREEs). The increasing demand for REEs is mainly driven by new technologies, and their current low recyclability has led them to become emer…
Plant growth promotion, metabolite production and metal tolerance of dark septate endophytes isolated from metal-polluted poplar phytomanagement sites
Numerous studies address the distribution and the diversity of dark septate endophytes (DSEs) in the literature, but little is known about their ecological role and their effect on host plants, especially in metal-polluted soils. Seven DSE strains belonging to Cadophora, Leptodontidium, Phialophora and Phialocephala were isolated from roots of poplar trees from metal-polluted sites. All strains developed on a wide range of carbohydrates, including cell-wall-related compounds. The strains evenly colonized birch, eucalyptus and ryegrass roots in re-synthesis experiments. Root and shoot growth promotion was observed and was both plant and strain dependent. Two Phialophora and Leptodontidium st…
Editorial: Exploring Plant Rhizosphere, Phyllosphere and Endosphere Microbial Communities to Improve the Management of Polluted Sites
International audience
Impact of poplar-based phytomanagement on soil properties and microbial communities in a metal-contaminated site
Despite a long history of use in phytomanagement strategies, the impacts of poplar trees on the structure and function of microbial communities that live in the soil remain largely unknown. The current study combined fungal and bacterial community analyses from different management regimes using Illumina-based sequencing with soil analysis. The poplar phytomanagement regimes led to a significant increase in soil fertility and a decreased bioavailability of Zn and Cd, in concert with changes in the microbial communities. The most notable changes in the relative abundance of taxa and operational taxonomic units unsurprisingly indicated that root and soil constitute distinct ecological microbi…
Combined omics approaches reveal distinct responses between light and heavy rare earth elements in Saccharomyces cerevisiae
International audience; The rapid development of green energy sources and new medical technologies contributes to the increased exploitation of rare earth elements (REEs). They can be subdivided into light (LREEs) and heavy (HREEs) REEs. Mining, industrial processing, and end-use practices of REEs has led to elevated environmental concentrations and raises concerns about their toxicity to organisms and their impact on ecosystems. REE toxicity has been reported, but its precise underlying molecular effects have not been well described. Here, transcriptomic and proteomic approaches were combined to decipher the molecular responses of the model organism Saccharomyces cerevisiae to La (LREE) an…
Inhabiting plant roots, nematodes, and truffles—polyphilus, a new helotialean genus with two globally distributed species
Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nu…
Torrefaction and pyrolysis of metal-enriched poplars from phytotechnologies: Effect of temperature and biomass chlorine content on metal distribution in end-products and valorization options
Abstract Torrefaction (290 °C) and pyrolysis (450 and 800 °C) trials were performed at pilot scale with a patented reactor on metal-enriched poplars from a contaminated soil managed by phytotechnologies and poplars cultivated on uncontaminated soil, for comparison. This study emphasized the influence of temperature on end-product yield, metal distribution in end-products and metal behavior. Results showed that the evolution of the end-product yield, i.e. biochar, bio-oil and gas fractions, was depending on temperature rather than other parameters such as the origin or metal content of the tested poplars. Torrefaction decreased the processed poplars weight, leading to metal-enriched torrefie…
Dendrochemical assessment of mercury releases from a pond and dredged-sediment landfill impacted by a chlor-alkali plant.
International audience; Although current Hg emissions from industrial activities may be accurately monitored, evidence of past releases to the atmosphere must rely on one or more environmental proxies. We used Hg concentrations in tree cores collected from poplars and willows to investigate the historical changes of Hg emissions from a dredged sediment landfill and compared them to a nearby control location. Our results demonstrated the potential value of using dendrochemistry to record historical Hg emissions from past industrial activities.
Spontaneous ecological recovery of vegetation in a red gypsum landfill: Betula pendula dominates after 10 years of inactivity
International audience; Red gypsum is the product of the neutralization of titanium dioxide (TiO 2) extraction residue from ilmenite and anatase. The disposal of red gypsum creates heterogeneous plots with layers that may include Fe, Ca, Al, Mg, Mn, S, and other elements and an alkaline pH that makes revegetation complicated and slow. The vertical and horizontal dispersion of the sediment particles are the main concern. Therefore, the establishment of precise vegetation cover is needed to address this issue. One of the aims of this study was (1) to explore the distribution of the spontaneous vegetation found along a red gypsum-formed landfill located at the Ochsenfeld site in eastern France…
Effect of mycorrhizal inoculation on metal accumulation by poplar leaves at phytomanaged sites
Abstract Phytotechnologies for the management of lands contaminated with potentially toxic elements (PTEs) are considered as gentle alternatives to conventional remediation techniques. During the last few years, phytotechnologies have progressively shifted to phytomanagement, a concept that includes the valorization of the plant biomass produced on the contaminated site. This study aimed at evaluating the mid-term effect of ecto- and endomycorrhizal inoculation on the reduction of PTE mobility in soils and foliar accumulation by two poplar clones, Skado ( Populus trichocarpa x P. maximowiczii ) and I-214 ( P. deltoides x P. nigra ), dedicated to bioenergy purposes. The effects of inoculatio…
Mercury uptake into poplar leaves
International audience; Tailings dumps require mercury stabilization to prevent air pollution by evaporated mercury, which can be achieved through plant covers. Plants are considered a net sink for atmospheric Hg via incorporation into leaf tissues. However, most studies related to Hg uptake by plants have considered plants exposed to only atmospheric Hg, whereas in the case of tailings dumps, plants are potentially exposed to both soil and atmospheric Hg. The goal of this work is to evaluate the relative contributions of root and atmospheric pathways by growing poplar (Populus trichocarpa X Populus maximowiczii/var Skado) cuttings on either control or polluted substrates and under either n…
Bacterial diversity associated with poplar trees grown on a Hg-contaminated site: Community characterization and isolation of Hg-resistant plant growth-promoting bacteria
International audience; Industrial waste dumps are rarely colonized by vegetation after they have been abandoned, indicating biological infertility. Revegetation of industrial tailings dumps is thus necessary to prevent wind erosion, metal leaching and has been shown to restore soil functions and ecosystem services. However, little is known about the microbial colonization and community structure of vegetated tailings following the application of restoration technologies. In this study, we investigated the rhizosphere and phyllosphere bacterial communities of a poplar tree plantation within a phytomanagement-based restoration program of a Hg-contaminated site. We used Illumina-based sequenc…
Evaluation of historical atmospheric pollution in an industrial area by dendrochemical approaches
International audience; We conducted a dendrochemical study in order to evaluate the exposure of territories and 19 populations to different types of pollutants and to characterise the history of pollution in one of the 20 most intensely industrialised areas of Europe: the industrial port zone of Fos, also heavily 21 urbanised. 22 To perform the study, two tree species have been selected, Pinus halepensis and Populus nigra, on arural plot located roughly 20 km away from the industrial harbor, an urban plot located in the city of Fos-sur-Mer and an industrial plot. Our study indicated that poplar was a more relevant model forthe dendrochemical studies exhibiting a higher bioaccumulation capa…
Inhabiting plant roots, nematodes, and truffles—Polyphilus, a new helotialean genus with two globally distributed species
Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nu…