0000000001317389
AUTHOR
Arantxa Rosado
showing 6 related works from this author
Additional file 2 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular chan…
2020
Additional file 2: Fig. S2. Evolutionary stability of a control system (E. coli transformed with plasmid pRHA27). Continuous fluorescence monitoring of four biological replicates (A-D) over one week.
Additional file 1 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular chan…
2020
Additional file 1: Fig. S1. Environmental robustness assessment of a control system (E. coli transformed with plasmid pRHA27; this system just expresses sfGFP under the control of the lac promoter). Fluorescence monitoring (normalized green fluorescence) when the culture medium changes (pH and temperature variation). Error bars correspond to standard errors over four biological replicates.
Boolean computation in plants using post-translational genetic control and a visual output signal
2018
[EN] Due to autotrophic growing capacity and extremely rich secondary metabolism, plants should be preferred targets of synthetic biology. However, developments in plants usually run below those in other taxonomic groups. In this work we engineered genetic circuits capable of logic YES, OR and AND Boolean computation in plant tissues with a visual output signal. The circuits, which are deployed by means of Agrobacterium tumefaciens, perform with the conditional activity of the MYB transcription factor Roseal from Antirrhinum majus inducing the accumulation of anthocyanins, plant endogenous pigments that are directly visible to the naked eye or accurately quantifiable by spectrophotometric a…
Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time
2020
Synthetic biology aims at engineering gene regulatory circuits to end with cells (re)programmed on purpose to implement novel functions or discover natural behaviors. However, one overlooked question is whether the resulting circuits perform as intended in variety of environments or with time. Here, we considered a recently engineered genetic system that allows programming the cell to work as a minimal computer (arithmetic logic unit) in order to analyze its operability regime. This system involves transcriptional and post-transcriptional regulations. In particular, we studied the analog behavior of the system, the effect of physicochemical changes in the environment, the impact on cell gro…
Additional file 3 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular chan…
2020
Additional file 3: Fig. S3. Schematics of the plasmids pRHA40, pRHA12, and pRHA1240.
Additional file 4 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular chan…
2020
Additional file 4: Dataset S1. Experimental and theoretical data of the half adder behavior. The Excel file contains an implementation of the mathematical model.