0000000001319864

AUTHOR

D. Thiele

showing 2 related works from this author

GIbPSs: a toolkit for fast and accurate analyses of genotyping-by-sequencing data without a reference genome.

2015

Genotyping-by-sequencing (GBS) and related methods are increasingly used for studies of non-model organisms from population genetic to phylogenetic scales. We present GIbPSs, a new genotyping toolkit for the analysis of data from various protocols such as RAD, double-digest RAD, GBS, and two-enzyme GBS without a reference genome. GIbPSs can handle paired-end GBS data and is able to assign reads from both strands of a restriction fragment to the same locus. GIbPSs is most suitable for population genetic and phylogeographic analyses. It avoids genotyping errors due to indel variation by identifying and discarding affected loci. GIbPSs creates a genotype database that offers rich functionality…

0301 basic medicineGeneticseducation.field_of_studyGenotyping TechniquesPopulationComputational BiologyLocus (genetics)Computational biologySequence Analysis DNABiology03 medical and health sciencesPhylogeography030104 developmental biologyGenetics PopulationGenotypeGeneticseducationIndelGenotypingGenotyping TechniquesEcology Evolution Behavior and SystematicsPaired-end tagBiotechnologyReference genomeMolecular ecology resources
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Data from: GIbPSs: a toolkit for fast and accurate analyses of genotyping-by-sequencing data without a reference genome

2016

Genotyping-by-sequencing (GBS) and related methods are increasingly used for studies of non-model organisms from population genetic to phylogenetic scales. We present GIbPSs, a new genotyping toolkit for the analysis of data from various protocols such as RAD, double-digest RAD, GBS, and two-enzyme GBS without a reference genome. GIbPSs can handle paired-end GBS data and is able to assign reads from both strands of a restriction fragment to the same locus. GIbPSs is most suitable for population genetic and phylogeographic analyses. It avoids genotyping errors due to indel variation by identifying and discarding affected loci. GIbPSs creates a genotype database that offers rich functionality…

medicine and health careMedicineRADpaired-end sequencingbioinformaticsLife sciences
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