0000000001325993
AUTHOR
Maria Rita Saladino
ANALISI DEI MECCANISMI ATTRAVERSO CUI RAS REGOLA LA PROLIFERAZIONE E L’APOPTOSI IN CELLULE DI ADENOCARCINOMA COLORETTALE HT-29
RAS is a family of small proteins with GTPase activity that regulate proliferation, differentiation and apoptosis in all cell types. The three major isoforms of RAS (H-, K- and N-RAS) differ only in the last 25 amino acids which are the site of different post-translational modifications that lead to diverse subcellular localization and efficiency of activation of alternative pathways of signal transduction. This might explain, at least in part, the different biological effects of the RAS isoforms in the cells. RAS mutations are a common event in tumorigenesis and in colorectal carcinomas the mutations in K-RAS are more frequent than mutations in H-RAS. In almost all cases, the genetic alter…
Analysis of the effects of K-RasG12V and K-RasG13D on the cell cycle
p21 Ras is small protein with GTPase activity that regulates proliferation, differentiation and apoptosis in all cell types. The three major isoforms of Ras (H-, K- and N-Ras) differing only for the last 24 aminoacids have different post-translational modifications that lead to localization in diverse plasma membrane microdomains and downstream activation of alternative pathways of signal transduction. This might explain, at least in part, the different biological effects of the Ras isoforms in the cells. Ras mutations are a common event in several tumours and in almost all cases they are point mutations in codons 12 or 13, and rarely in codon 61. These mutations lead to a constitutively ac…