0000000001334742
AUTHOR
Pastorella, Gabriele
showing 3 related works from this author
Metodi genetici per migliorare il biorisanamento di siti contaminati da Cr(VI)
2008
non disponibile
Genetic technology to improve biofilms electroactivity for bioremediation applications
2010
Hexavalent chromium bioremediation occurs through its reduction to Cr(III) and its following precipitation. Geobacter metallireducens is a dissimilatory metal reducing bacteria known to reduce Cr(VI) to Cr(III) through extracellular electron transfer. However, the effectiveness of this bioreduction process is limited by the low toxicity resistance of G. metallireducens to Cr(VI). G. metallireducens mutants with improved Cr(VI) toxicity resistance can be obtained by genome shuffling method. Here we present the experimental setup for genome shuffling of G. metallireducens, including the optimization of mutagenesis procedure and the respiration rate measure for G. metallireducens in potentiost…
GEOBACTER METALLIREDUCENS MUTANTS FOR CHROMIUM BIOREMEDIATION
2011
Geobacter metallireducens is known to use oxidized metals such as Fe(III) and Mn(IV) as terminal extracellular electron acceptors. G. metallireducens can reduce the highly soluble and toxic Cr(VI) to its insoluble form Cr(III), thus enabling chromium removal from contaminated water and soil. This process is limited by the low toxicity resistance of G. metallireducens to Cr(VI). Genome shuffling can generate mutants with improved phenotype. In our work we are carrying out G. metallireducens genome shuffling, in order to increase its resistance to Cr(VI). In Geobacter species, metal toxicity resistance is correlated to the respiration rate, hence to the reduction rate of the metallic electron…