Proceedings of the ASPA 18th Congress, Palermo, June 9-12, 2009

2009

Effects of Beclomethasone dipropionate and Formoterol in reducing oxidative stress induced by cigarette smoke extracts and IL-17

2011

Rationale: Oxidative stress is involved in airway inflammatory diseases. Inhaled-corticosteroids reduce airway inflammation and the combination with long-acting β2 agonists enhances this effect. Objective: to investigate whether Cigarette smoke extracts (CSE) and Interleukin-17A (IL-17A) activate airway epithelial cells to release markers of oxidative/nitrosative stress and to investigate the effect of beclomethasone dipropionate (BDP) and formoterol. Methods: Human bronchial epithelial cells (16HBE) were stimulated with different concentrations of CSE (from 0 to 10%) to evaluate the expression of IL-17 receptor (IL-17R). 16HBE were also stimulated with CSE (2.5%) with and withouth rhIL-17A…

β2long-acting and anticholinergic drugs synergistically control TGFβ1-mediated neutrophilic inflammation in COPD: an “in vitro” model.

2011

TGFβ1 is involved in airway inflammation in COPD and in the regulation of muscarinic receptors (mAChRs) expression. We quantified TGFβ1 and Acetylcholine (ACh) concentrations in induced sputum supernatants (ISs) from 10 Healthy Controls (HC), 14 Healthy Smokers (HS) and 16 COPDs. We tested: 1) ISs from HC, HS and COPD on neutrophil adhesion to human bronchial epithelial cells (16HBE) in the presence or absence of an anti-TGFβ1ab or Olodaterol (β2-adrenoceptor agonist) and Tiotropium (Spiriva®) alone or in combination; 2) ISs from COPD on mAChRs expression in 16HBE in the presence or absence of anti-TGFβ1-antibody and Olodaterol; 3) hrTGFβ1on neutrophil adhesion and mChRs and ChAT expression…

Role of non cholinergic system components in Chronic Rhinosinusitis and Nasal Polyps

2010

The Choline acetyltransferase (ChAT) enzyme and its product Acetylcholine (ACh) are classically components of the cholinergic system, however recent data clearly demonstrated their potential role in the bronchial airway inflammation. The aim of this study was to identify whether ChAT, Muscarinic receptor M3 and ACh are involved in the nasal airway inflammation of subjects with Chronic Rhinosinusitis (CR) and Nasal polyps (common in many patients with CR). We collected nasal specimens from normal subjects, from allergic patients with CR (ACR) and from ACR and nasal polyps. Fragments from surgical biopsies were extracted for both total mRNA and total proteins to analyse the levels of ChAT and…