0000000001335986
AUTHOR
Siena, L
showing 2 related works from this author
β2long-acting and anticholinergic drugs synergistically control TGFβ1-mediated neutrophilic inflammation in COPD: an “in vitro” model.
2011
TGFβ1 is involved in airway inflammation in COPD and in the regulation of muscarinic receptors (mAChRs) expression. We quantified TGFβ1 and Acetylcholine (ACh) concentrations in induced sputum supernatants (ISs) from 10 Healthy Controls (HC), 14 Healthy Smokers (HS) and 16 COPDs. We tested: 1) ISs from HC, HS and COPD on neutrophil adhesion to human bronchial epithelial cells (16HBE) in the presence or absence of an anti-TGFβ1ab or Olodaterol (β2-adrenoceptor agonist) and Tiotropium (Spiriva®) alone or in combination; 2) ISs from COPD on mAChRs expression in 16HBE in the presence or absence of anti-TGFβ1-antibody and Olodaterol; 3) hrTGFβ1on neutrophil adhesion and mChRs and ChAT expression…
Role of non cholinergic system components in Chronic Rhinosinusitis and Nasal Polyps
2010
The Choline acetyltransferase (ChAT) enzyme and its product Acetylcholine (ACh) are classically components of the cholinergic system, however recent data clearly demonstrated their potential role in the bronchial airway inflammation. The aim of this study was to identify whether ChAT, Muscarinic receptor M3 and ACh are involved in the nasal airway inflammation of subjects with Chronic Rhinosinusitis (CR) and Nasal polyps (common in many patients with CR). We collected nasal specimens from normal subjects, from allergic patients with CR (ACR) and from ACR and nasal polyps. Fragments from surgical biopsies were extracted for both total mRNA and total proteins to analyse the levels of ChAT and…