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RESEARCH PRODUCT
Identification of 5,6,7,8-tetrahydropterin and 5,6,7,8-tetrahydrobiopterin in Drosophila melanogaster.
Juan J. GuillamónJuan Ferrésubject
GeneticsbiologyMutantBiophysicsWild typeCell BiologyElectrochemical detectionTetrahydrobiopterinbiology.organism_classificationKidneyBiochemistryHigh-performance liquid chromatographyBiopterinPterinsRatsDrosophila melanogasterBiochemistryIn vivomedicineAnimalsDrosophila melanogasterMolecular BiologyChromatography High Pressure Liquidmedicine.drugdescription
Summary Using reversed-phase high-performance liquid chromatography with electrochemical detection we have demonstrated the occurrence of 5,6,7,8-tetrahydropterin and 5,6,7,8-tetrahydrobiopterin in Drosophila melanogaster . The former is the first time that has been detected in vivo . The identification has been based on the retention times, hydrodinamic voltagrams and the differential concentration in three strains of Drosophila melanogaster . Compared to the wild type, the Punch 2 mutant has diminished levels of both pteridines, whereas Henna-recessive 3 lacks completely tetrahydropterin and has increased levels of tetrahydrobiopterin, as expected according to their biochemical lesions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-04-01 | Biochemical and biophysical research communications |