6533b7cefe1ef96bd125719b

RESEARCH PRODUCT

Glomerular basement membrane: evidence for collagenous domain of the alpha 3 and alpha 4 chains of collagen IV.

Billy G. HudsonWilliam R. FaggJoaquín TimonedaCharles E. SchwartzMilton E. NeolkenJan P.m. Langeveld

subject

Basement membraneGel electrophoresischemistry.chemical_classificationChemistryRenal glomerulusMacromolecular SubstancesProtein ConformationProtein subunitGlomerular basement membraneKidney GlomerulusBiophysicsBiological membraneCell BiologyBiochemistryBasement Membranemedicine.anatomical_structureBiochemistryDomain (ring theory)medicineAnimalsCattleCollagenAmino AcidsGlycoproteinMolecular Biology

description

Abstract A collagenous component(s) of Mr = 60K was extracted from glomerular basement membrane with urea and was purified. Upon digestion, it yielded a collagenase-resistant fragment(s) of Mr = 23.5K. Both component and fragment showed immunochemical identity with the noncollagenous domains of the new α3 & α4 chains of collagen IV. The component is characterized by a collagenous domain of about 280 residues and a noncollagenous domain of about 250 residues. These findings further establish these new chains as distinct entities of collagen IV.

yearjournalcountryeditionlanguage
1990-07-01Biochemical and biophysical research communications
10.1016/0006-291x(90)91277-yhttps://pubmed.ncbi.nlm.nih.gov/2372295