6533b7cefe1ef96bd1257a3c

RESEARCH PRODUCT

Clickable poly-L-lysine for the formation of biorecognition surfaces

Almudena MartíSander KoemanDaniele Di IorioJurriaan Huskens

subject

chemistry.chemical_classificationChemistryGeneral Chemical EngineeringBiomoleculeLysineADN02 engineering and technologyGeneral ChemistryPolymer010402 general chemistry021001 nanoscience & nanotechnology01 natural sciencesCombinatorial chemistryPolyelectrolyte0104 chemical sciencesReaction rateAdsorptionBiomolèculesCovalent bondClick chemistry0210 nano-technology

description

Biomolecules are immobilized onto surfaces employing the fast and stable adsorption of poly-l-lysine (PLL) polymers and the versatile copper-free click chemistry reactions. This method provides the combined advantages of versatile surface adsorption with density control using polyelectrolytes and of the covalent and orthogonal immobilization of biomolecules with higher reaction rates and improved yields of click chemistry. Using DNA attachment as a proof of concept, control over the DNA probe density and applicability in electrochemical detection are presented.

yearjournalcountryeditionlanguage
2019-11-04
10.1039/c9ra08714ahttps://doi.org/10.1039/C9RA08714A