6533b7cefe1ef96bd1257bf9

RESEARCH PRODUCT

Improved resolution power of electrophoretic fractionation of DNA using a voltage gradient up and down application

Laura PalmeriRosalia SanfilippoRainer BarbieriGiorgia SisinoDaniele Bellavia

subject

Free-flow electrophoresisGel electrophoresisElectrophoresisDNA fractionationChromatographyGel electrophoresis of nucleic acidsResolution (mass spectrometry)Voltage gradient gel electrophoresisChemistryClinical BiochemistryAnalytical chemistryFractionationDNABiochemistryELECTROPHORESIS INSTRUMENTATIONAnalytical Chemistrychemistry.chemical_compoundElectrophoresisIndicators and ReagentsDNA

description

The improved resolution power of electrophoretic fractionation of DNA in a wide range of molecular masses is demonstrated using an "up and down" application of voltage gradient gel electrophoresis (VGGE). This application also allows separation of different DNA fragments which are poorly fractionated in conventional electrophoresis.

yearjournalcountryeditionlanguage
2004-09-07
http://hdl.handle.net/10447/16284