6533b7cffe1ef96bd1257c88
RESEARCH PRODUCT
Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices
Antonio Abad-fuentesConsuelo AgullóAntonio Abad-somovillaJosep V. MercaderFrancesc A. Esteve-turrillassubject
Detection limitcELISAChromatographybiologymedicine.drug_classChemistryFungicideGeneral Chemical EngineeringGeneral EngineeringFludioxonilMonoclonal antibodyAnalytical ChemistryFungicidePolyclonal antibodiesbiology.proteinmedicineFruit juicesFudioxonilMode of actionSpecific enzymedescription
Fludioxonil is a fungicide with a singular mode of action that is widely employed in the treatment of fruit crops. A competitive enzyme-linked immunosorbent assay (cELISA) has been developed and validated for the determination of residues of this fungicide in fruit juices. A collection of monoclonal antibodies (mAbs) against the fungicide fludioxonil has been produced and characterized using direct and indirect cELISAs. The high affinity achieved (IC50lower than 0.5 μg L-1) considerably improved that obtained with previous polyclonal antibodies. The proposed cELISA was applied to the analysis of fludioxonil residues in apple juice and red grape must samples with a limit of quantification of 5 and 10 μg L-1, respectively. A validation of the method has been performed in terms of sensitivity, selectivity, trueness, precision, and robustness. Finally, blind-spiked samples were measured by the proposed cELISA and by a gas chromatography-mass spectrometry (GC-MS) method, with equivalent results by both methodologies.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 | Anal. Methods |