Gripas vīrusa hemaglutinīna fragmenta strukturāla izpēte un eksponēšana uz vīrusiem līdzīgo daļiņu virsmas

6533b7cffe1ef96bd1257f2e

RESEARCH PRODUCT

Gripas vīrusa hemaglutinīna fragmenta strukturāla izpēte un eksponēšana uz vīrusiem līdzīgo daļiņu virsmas

Anna Kiršteina

subject

rentgenstaru kristalogrāfija vīrusiem līdzīgās daļiņas ķīmiskā konjugēšana Bioloģija hemaglutinīna stalks gripas vīruss

description

Iekļaujot vakcīnā konservatīvus vīrusa proteīnu domēnus, būtu iespējams nodrošināt plaša spektra aizsardzību pret dažādiem gripas vīrusu apakštipiem. Viens no šādiem konservatīviem rajoniem ir hemaglutinīna (HA) stalka peptīds HA2.3. Bakalaura darba mērķis bija noteikt HA2.3 struktūru, tā eksponēšanas iespējas uz dažādu vīrusiem līdzīgo daļiņu (VLP) virsmas, un pētīt šo daļiņu imunoloģiskās īpašības. Pētījumā tika veikta HA2.3 ekspresija, attīrīšana, kristalizācija. Izmantojot rentgenstaru difrakciju, tika noteikts, ka HA2.3 struktūra vides pH 5,5 atbilst natīvajai HA stalka trimēra post fusion konformācijai. HA2.3 tika ķīmiski konjugēts pie dažādu VLP virsmas, kuras pēc tam izmantoja peļu imunizācijai. Himēro VLP izraisītā antivielu atbilde pret HA2.3 bija zemāka nekā HA2.3 kontrolei, kas, visticamāk, izskaidrojams ar HA2.3 konformācijas izmaiņām.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/28577

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