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RESEARCH PRODUCT

Label-Free Proteomics of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry

Stefan TenzerMalte SielaffUte Distler

subject

0303 health sciencesComputer science030302 biochemistry & molecular biologyQuantitative proteomicsMass spectrometryProteomicsIon03 medical and health sciencesLabel-free quantificationSample preparationData-independent acquisitionBottom-up proteomicsBiological system030304 developmental biology

description

Over the past two decades, unbiased data-independent acquisition (DIA) approaches have gained increasing popularity in the bottom-up proteomics field. Here, we describe an ion mobility separation enhanced DIA workflow for large-scale label-free quantitative proteomics studies where starting material is limited. We set a special focus on the single pot solid-phase-enhanced sample preparation (SP3) protocol, which is well suited for the processing of quantity-limited samples.

yearjournalcountryeditionlanguage
2021-01-01
https://doi.org/10.1007/978-1-0716-1024-4_23