6533b7cffe1ef96bd1258d24
RESEARCH PRODUCT
Sensitive determination of probenecid in urine samples by reversed-phase liquid chromatography and UV-visible detection using solid-phase extraction techniques for sample clean-up
Pilar Campíns-falcóAdela Sevillano-cabezaRosa Herráez-hernándezsubject
Detection limitChromatographyChemistryOrganic ChemistryClinical BiochemistryExtraction (chemistry)Reversed-phase chromatographyUrineBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryProbenecidPhase (matter)medicineSolid phase extractionmedicine.drugdescription
This study describes a rapid method for the determination of probenecid in human urine by liquid chromatography with UV detection at 254 nm, after clean-up through a C8 solid-phase extraction column. Liquid chromatography was carried out on a C18-bonded phase using an acetonitrile-acetate buffer (pH=4) gradient elution. Ethacrynic acid was used as internal standard. The system has been applied to the determination of probenecid in the 0.10–100.0 μg/ml concentration range; the limit of detection was 5 ng/mL.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-03-01 | Chromatographia |