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RESEARCH PRODUCT
Size Exclusion Chromatography of Proteins on Improved Bonded Silica Columns
K.k. UngerM. Chatziathanassiousubject
chemistry.chemical_classificationHydrophobic effectChromatographychemistryResolution (mass spectrometry)Phase (matter)Size-exclusion chromatographyHigh resolutionPolymerProtein chemistryHigh-performance liquid chromatographydescription
Publisher Summary This chapter discusses the size exclusion chromatography (SEC) of proteins on improved bonded silica columns. Over the past few years, the use of high performance liquid chromatography (HPLC) has been extended to protein chemistry as a powerful separation and isolation technique. A spectrum of variants providing high resolution potential—ion exchange, reversed phase, hydrophobic interaction, affinity, and size exclusion—is available. Of these, SEC is the simplest with respect to method development and equipment design: proteins are separated according to their size in a short and predictable time span under isocratic conditions. The SEC columns are packed with micro-particulate hydrophilic bonded silicas or hydrophilic cross-linked polymer gels and are capable of resolving 10 to 20 proteins with high mass recoveries and maintenance of biological activity in an acceptable analysis time. Recent studies indicate that SEC resolution is open to further improvement by employing specifically tailored packings and operating the columns under appropriate conditions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1985-01-01 |