Cilvēka asins mikrobioma kompozīcijas, stabilitātes un izcelsmes noteikšana veselos indivīdos

6533b7cffe1ef96bd12593b2

RESEARCH PRODUCT

Cilvēka asins mikrobioma kompozīcijas, stabilitātes un izcelsmes noteikšana veselos indivīdos

Maija Rozenberga

subject

asins mikrobioms mikrobioma izcelsme 16S rRNS mikrobioma stabilitāte Bioloģija

description

Neseni pētījumi ir apliecinājuši asins mikrobioma klātesamību veselos cilvēkos, tomēr tā izcelsme vēl ir neskaidra. Maģistra darba mērķis bija noskaidrot asins mikrobioma kompozīciju, potenciālo izcelsmi un dinamiku laika gaitā veseliem indivīdiem. Asins mikrobioma izcelsmes noskaidrošanai 10 veseliem indivīdiem ievāca pilnasins paraugus un ādas, maksts, mutes un fēču mikrobioma uztriepes. Pilnasins un fēču paraugus ievāca no diviem laika punktiem ar 3 mēnešu intervālu. Ar 16S rRNS gēna V3-V4 reģiona analīzi noteica to taksonomisko sastāvu. Noskaidrojās, ka asins mikrobioms ir unikāls - tajā izteikti dominē Proteobacteria tipa Pseudomonas ģints pārstāvji, un vienlaikus tā potenciālā izcelsme daļēji varētu būt no ādas mikrobioma. Pētījuma dalībniekiem 3 mēnešu laika periodā asins un fēču mikrobioms saglabāja stabilitāti. Darbs izstrādāts no 06.2020. līdz 05.2021. Latvijas Biomedicīnas pētījumu un studiju centrā.

year	journal	country	edition	language
2021-01-01

https://dspace.lu.lv/dspace/handle/7/54491

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SHSocial Sciences and Humanities
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