6533b7cffe1ef96bd12597ce
RESEARCH PRODUCT
The Influence of the Proton Gradient on the Activation of Ferredoxin-NADP+-oxidoreductase by Light
Roland PschornWolfgang RühleAloysius Wildsubject
ChemistryBiophysicsLight activationElectrochemical gradientElectron transport chainGeneral Biochemistry Genetics and Molecular BiologyFerredoxin:NADP+ oxidoreductasedescription
Ferredoxin-NADP+-oxidoreductase (FNR, EC 1.18.1.2) has been shown to be activated by light within a few seconds during dark-light transitions and inactivated in the dark. In previous papers this could be pointed out by the correlation of cytochrome f induction kinetics to the rate of NADP-photoreduction and the variable fluorescence. The present study deals with the role of the proton gradient during the activation process. The transition from an inactive to an active form is followed continuously in an in situ system. The steady-state rate of NADP-photoreduction is affected only by ionophores which inhibit a formation of the proton gradient, but not by inhibitors of the electric field. It correlates to the 9-aminoacridine fluorescence quench and the light scattering signals at 535 nm. The perception of the pH-gradient through the enzyme is still a matter of discussion.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-04-01 | Zeitschrift für Naturforschung C |