6533b7cffe1ef96bd1259b1c

RESEARCH PRODUCT

Silver Impregnation of Alzheimer's Neurofibrillary Changes Counterstained for Basophilic Material and Lipofuscin Pigment

Eva BraakJürgen BohlThomas G. OhmHeiko Braak

subject

Pathologymedicine.medical_specialtySilverALIZARIN REDLipofuscinLipofuscinSilver stainPigmentsymbols.namesakechemistry.chemical_compoundAlzheimer DiseasemedicineHumansPerformic acidStaining and LabelingChemistryMiddle AgedStainingBasophilicnervous systemNissl Bodiesvisual_artNeurofibrilsvisual_art.visual_art_mediumNissl bodysymbolsFemalesense organsAnatomy

description

A method is described in which selective silver staining of Alzheimer's neurofibrillary changes is combined with staining of cell nuclei, Nissl material, and lipofuscin granules. Formalin fixed, paraffin embedded sections of human autopsy tissue are silver stained according to a method proposed by Gallyas. Lipofuscin is stained by crotonaldehyde fuchsin following performic acid oxidation. Nissl substance is visualized by either Darrow red or gallocyanin-chrome alum staining. Architectonic units showing the specific pathology and the neuronal types prone to develop the neurofibrillary changes can be recognized using this technique.

https://doi.org/10.3109/10520298809107184