6533b7d0fe1ef96bd125a2d4
RESEARCH PRODUCT
Proton-dependent kinetics of citrate uptake in growing cells ofLactococcus lactissubsp.lactisbv.diacetylactis
Sidonie DanielCharles DivièsRémy Cachonsubject
chemistry.chemical_classificationPermeaseKineticsLactococcus lactisCitrate transportBiologyMembrane transportbiology.organism_classificationStreptococcaceaeMicrobiologyDivalentchemistryBiochemistryGeneticsMolecular BiologyTernary complexdescription
The kinetic analysis of citrate uptake in growing cells of Lactococcus lactis subsp. lactis biovar. diacetylactis identified a proton-dependent transport and suggested the divalent anionic species as the form of citrate transported across cell membranes. The reaction followed Michaelis-Menten kinetics for a two-substrate reaction. The limiting steps were the formation of the ternary complex and the rate of transport. Temperature modified the activity of the permease, increasing the uptake rate.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-09-01 | FEMS Microbiology Letters |