6533b7d0fe1ef96bd125a3ab

RESEARCH PRODUCT

Derivatization of ephedrine with o-phthaldialdehyde for liquid chromatography after treatment with sodium hypochlorite.

Rosa Herráez-hernándezPilar Campíns-falcó

subject

EphedrineChromatographySodium HypochloriteOrganic ChemistryGeneral MedicineBiochemistryHigh-performance liquid chromatographyFluorescence spectroscopyAnalytical ChemistryO-Phthalaldehydechemistry.chemical_compoundchemistrySodium hypochloritemedicineSample preparationEphedrineDerivatizationDerivative (chemistry)o-Phthalaldehydemedicine.drugChromatography Liquid

description

The usefulness of the reaction with NaClO followed by derivatization with o-phthaldialdehyde (OPA) and N-acetyl-L-cysteine (NAC) has been investigated for the chromatographic analysis of ephedrine. The influence of parameters affecting the two-stage reaction has been evaluated, including concentration of NaClO, time of reaction, temperature and pH. On the basis of these studies, conditions for the pre-column and (automated) post-column determination of ephedrine are presented. The described conditions have been applied to the measurement of ephedrine in the concentration intervals 0.2-20.0 microg/ml and 2.0-50.0 microg/ml for the pre-column and post-column methods, respectively. The possibility of applying the NaClO/ OPA-NAC method to other primary, secondary and tertiary derivative amphetamines has also been evaluated.

yearjournalcountryeditionlanguage
2000-10-24Journal of chromatography. A
10.1016/s0021-9673(00)00687-7https://pubmed.ncbi.nlm.nih.gov/11043588