6533b7d0fe1ef96bd125a467
RESEARCH PRODUCT
Fluorescence of soil humic acids and their fractions obtained by tandem size exclusion chromatography-polyacrylamide gel electrophoresis
Ghislain GuyotClaire RichardOlga TrubetskayaOleg TrubetskojFrancis Andreuxsubject
Gel electrophoresis[SDE] Environmental SciencesChromatographyChemistry[SDV]Life Sciences [q-bio]Size-exclusion chromatographyMineralogyFluorescenceFluorescence spectroscopy[SDV] Life Sciences [q-bio]Electrophoresischemistry.chemical_compoundGeochemistry and Petrology[SDE]Environmental SciencesCarboxylatePolyacrylamide gel electrophoresisChernozemdescription
Humic acids (HAs) extracted from soils of different origin (chernozem, ferralsol and ranker) and their fractions (A, B and C+D) obtained by tandem size exclusion chromatography–polyacrylamide gel electrophoresis were investigated by steady-state fluorescence spectroscopy in the emission mode. Independently of HA source, high molecular size fractions A and B are shown to be weakly fluorescent. The main fluorophores, especially those emitting at long wavelength (around 500–510 nm), are contained in the polar and low molecular size fractions C+D. As indicated by the observed pH effect, aromatic structures bearing carboxylate and OH substituents may be involved in these longer wavelength emissions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-03-01 |