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RESEARCH PRODUCT
RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase
Normunds LicisElita Avotasubject
chemistry.chemical_compoundbiologychemistryBiochemistryTranscription (biology)RNA editingRNA polymerasebiology.proteinRNA polymerase IRNA-dependent RNA polymeraseRNARNA polymerase IIPolymerasedescription
The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-01-01 |