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RESEARCH PRODUCT
Enhancement of brain choline levels by nicotinamide: mechanism of action
Christina ErbJochen Kleinsubject
MaleNiacinamidemedicine.medical_specialtyTime FactorsInjections Subcutaneouschemistry.chemical_elementIn Vitro TechniquesCalciumHippocampusCholinechemistry.chemical_compoundPhospholipase A2Internal medicinemedicineAnimalsCholineRats WistarPhospholipase AbiologyNicotinamideGeneral NeuroscienceBrainNADRatsB vitaminsEndocrinologyLiverchemistryNiacinamidebiology.proteinNAD+ kinasedescription
Following the subcutaneous (s.c.) administration of nicotinamide (10 mmol/kg), the brain and CSF levels of nicotinamide were increased to millimolar concentrations, but the concentrations of N-methylnicotinamide (NMN) in the CSF, and of NMN and NAD+ in brain tissue were not significantly altered. Concomitantly, nicotinamide caused increases of the choline levels in the venous brain blood. In hippocampal slices, nicotinamide (1-10 mM) induced choline release in a calcium- and mepacrine-sensitive manner and, in [3H]choline-labelled slices, increased the levels of [3H]lyso-phosphatidylcholine and [3H]glycerophosphocholine. We conclude that nicotinamide enhances brain choline concentrations by mobilising choline from choline-containing phospholipids, presumably via activation of phospholipase A2, while the formation of NMN does not contribute to this effect.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-06-01 | Neuroscience Letters |