6533b7d0fe1ef96bd125b8ad

RESEARCH PRODUCT

Analysis of meat samples for anabolic steroids residues by liquid chromatography/tandem mass spectrometry.

Cristina BlascoCarlos Van PeteghemChristof Van Poucke

subject

ElectrospraySpectrometry Mass Electrospray IonizationMeatSwineElectrospray ionizationMass spectrometryTandem mass spectrometryBiochemistryHigh-performance liquid chromatographySensitivity and SpecificityPoultryAnalytical ChemistryAnabolic AgentsLiquid chromatography–mass spectrometryTandem Mass SpectrometryAnimalsSample preparationSolid phase extractionChromatographyChemistryMusclesOrganic ChemistryUncertaintyGeneral MedicineCattleChromatography Liquid

description

A rapid, specific and highly sensitive multi-residue method for the determination of anabolic steroid residues in bovine, pork and poultry muscle tissues was developed. The sample preparation involves enzymatic digestion followed by extraction with methanol. The crude extract was cleaned up by solid-phase extraction (SPE) combining C18 and NH2 columns. The detection was carried out by a highly sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method using both positive and negative ionization modes. Natural and synthetic steroids covering different polarities could be extracted, concentrated and purified using one single method. Mobile phase composition and additives were optimized to achieve the highest sensitivity. The linearity was not good enough for quantitative analysis but the method was well-suited for qualitative confirmation. The method was validated according to the European Commission Decision 2002/657/EC. Decision limits (CCalpha) and detection capabilities (CCbeta) were below 0.5 ng g(-1) for all the compounds in the three types of meat studied. The developed method is suitable for routine analysis in our laboratories.

yearjournalcountryeditionlanguage
2007-06-01Journal of chromatography. A
10.1016/j.chroma.2007.03.090https://pubmed.ncbi.nlm.nih.gov/17459396