6533b7d0fe1ef96bd125b8d6
RESEARCH PRODUCT
Effect of the cytochrome P-450 inactivator 1-aminobenzotriazole on the metabolism of chlortoluron and isoproturon in wheat
F. CabanneRené ScallaF. DurstP. GaillardonD. Hubysubject
0106 biological sciencesCytochromeStereochemistry[SDV]Life Sciences [q-bio]Health Toxicology and Mutagenesis01 natural sciencesHydroxylationchemistry.chemical_compoundComputingMilieux_MISCELLANEOUSDemethylationchemistry.chemical_classificationbiologyChemistryfood and beveragesCytochrome P45004 agricultural and veterinary sciencesGeneral MedicineMetabolismMonooxygenase[SDV] Life Sciences [q-bio]EnzymeBiochemistryChlortoluron040103 agronomy & agriculturebiology.protein0401 agriculture forestry and fisheriesAgronomy and Crop Science010606 plant biology & botanydescription
Abstract Roots of young wheat plants ( Triticum aestivum cv Clement) were treated with [ 14 C]chlortoluron or [ 14 C]isoproturon alone or mixed with 1-aminobenzotriazole (ABT), a mechanism-based inactivator of cytochrome P -450 monooxygenases. Radioactivity extracted from shoots slightly decreased during periods of metabolism, this decrease being reduced by ABT in the case of isoproturon. ABT strongly inhibited the metabolism of both herbicides. Accumulation of metabolites was generally depressed in the presence of ABT; however, levels of the free N -monodemethylated derivatives were little or not affected. It is concluded that ABT is a synergist of chlortoluron and isoproturon in wheat because it inhibits the enzymes responsible for the ring-alkyl hydroxylation of chlortoluron and putatively of isoproturon, as well as the second N -demethylation of isoproturon. The possibility that those enzymes belong to the class of cytochrome P -450 monooxygenases is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-07-01 |