Zarnu epitēlija proliferācijas marķieru analīze mezenteriālās zarnu išēmijas modelī in vivo

6533b7d1fe1ef96bd125c6c0

RESEARCH PRODUCT

Zarnu epitēlija proliferācijas marķieru analīze mezenteriālās zarnu išēmijas modelī in vivo

Elīza ŽūKure

subject

Lgr5 zarnu cilmes šūnu marķieris Farmācija kvantitatīvā polimerāzes ķēdes reakcijas (qPĶR) metode Ki67 šūnu proliferācijas marķieris mezenteriālā išēmija

description

Mezenteriālā išēmija ir retāk sastopama slimība ar augstiem mirstības rādītajiem. Zarnas ir visjutīgākais orgāns pret išēmijas izraisītiem bojājumiem. Akūta mezenteriāla išēmija rodas, ja zarnas netiek nodrošinātas ar pietiekamu skābekļa daudzumu. Šīs slimības diagnoze var būt sarežģīta, jo simptomi ir līdzīgi citu slimību patoloģijām un slimības stāvoklis parasti strauji progresē. Mezenteriālās išēmijas identificēšanai var izmantot ģenētiskos marķierus.Maģistra darba pētījuma mērķis bija izvērtēt šūnu proliferācijas marķiera Ki67 un zarnu cilmes šūnu marķiera Lgr5 ekspresiju žurku zarnu išēmijas modelī ar kvantitatīvo polimerāzes ķēdes reakcijas metodi. Analizējot Ki67 un Lgr5 gēnu ekspresijas datus, netika uzrādīts statistiskais nozīmīgums, taču salīdzinot gēnu ekspresiju ar kontroles grupu, Lgr5 gēns uzrāda ekspresijas izmaiņas.

year	journal	country	edition	language
2022-01-01

https://dspace.lu.lv/dspace/handle/7/59780

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