Retrotranspozonu struktūra parastās priedes (Pinus sylvestris L.) genomā un to ekspresija

6533b7d1fe1ef96bd125d3b8

RESEARCH PRODUCT

Retrotranspozonu struktūra parastās priedes (Pinus sylvestris L.) genomā un to ekspresija

Angelika Voronova

subject

retrotranspozoni diferenciāla ekspresija gymnosperms Molecular biology Scots pine (Pinus sylvestris L.)kailsēkļi Dzīvās dabas zinātnes Bioloģija Transposable elements Molekulārā bioloģija Transponējamie elementi retrotransposons differential expression

description

ANOTĀCIJA Promocijas darbs izstrādāts Latvijas Valsts mežzinātnes institūtā „Silava”. Pētījuma mērķis bija identificēt aktīvos retrotranspozonus parastās priedes (Pinus sylvestris L.) genomā un raksturot to transkripcijas līmeni dažādu stresa veidu ietekmē kontrolētos apstākļos. Pētījumā izmantoti nespecifiskie retrotranspozonu marķieri balstīti uz augsti konservatīvām tRNS piesaistes vietām. Viena klona priežu rametus pakļāva dabā izplatītam karstuma stresam, kukaiņu invāzijai (priežu hermess (Pineus pini L.), kā arī mākslīgi izraisītai augu hormonu (abscīzskābes un salicilskābes) ietekmei. Darba rezultātā identificēti pārsvarā I klases mobīlo elementu pārstāvji, kā arī tos saturošie himēriskie transkripti. Parādīts, ka retrotranspozonu sekvences tiek ekspresētas pētamajos stresa apstākļos. Analizēta identificēto sekvenču izplatība priedes un 60 citu kailsēkļu sugu genomos. Identificēts un aprakstīts iespējams transpozīcijas gadījums priedes rametiem. Atslēgvārdi: Transponējamie elementi, retrotranspozoni, parastā priede (Pinus sylvestris L.), kailsēkļi, diferenciāla ekspresija.

year	journal	country	edition	language
2014-01-01

https://dspace.lu.lv/dspace/handle/7/3678

PEPhysical Sciences and Engineering
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