6533b7d1fe1ef96bd125d4ed
RESEARCH PRODUCT
A new method to isolate lichen algae by using percoll® gradient centrifugation
Angeles CalatayudEva BarrenoAlfredo GuéraSimón Fossubject
Algal cellsPhotoinhibitionAlgaebiologyEvernia prunastriBotanyPercoll gradient centrifugationLichenbiology.organism_classificationEcology Evolution Behavior and SystematicsRamalina farinaceaThallusdescription
AbstractA rapid method to isolate intact functional algae from the lichens Evemia prunastri and Ramalina farinacea has been developed. This method is based on the use of Percoll® gradients after mechanical disruption of lichen thalli. Results obtained show that the algal preparations were virtually free of contamination by fungal hyphae. The purified algal cells were photosynthetically active and without symptoms of photoinhibition, which indicates their functional integrity. This method may be used for the isolation of intact algae from a broad range of lichen species.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-07-01 | The Lichenologist |