6533b7d1fe1ef96bd125d6ce
RESEARCH PRODUCT
Enhancerless Cytomegalovirus Is Capable of Establishing a Low-Level Maintenance Infection in Severely Immunodeficient Host Tissues but Fails in Exponential Growth
Katja C. ErlachAnnette FinkMatthias J. ReddehaseRares PinteaAna AnguloKai A. KroppElena IsernNiels A. W. LemmermannJürgen PodlechPeter Ghazalsubject
Gene Expression Regulation ViralMutantImmunology/dk/atira/pure/subjectarea/asjc/2400/2406CytomegalovirusMice SCIDBiologyMicrobiologyVirusImmunocompromised HostMiceExponential growthIn vivoVirologyAnimalsHumans/dk/atira/pure/subjectarea/asjc/2400/2403EnhancerTranscription factorMice Inbred BALB CVirologyGenome Replication and Regulation of Viral Gene ExpressionEnhancer Elements GeneticInsect ScienceCytomegalovirus InfectionsHost-Pathogen InteractionsCytomegalovirus infectionsdescription
ABSTRACT Major immediate-early transcriptional enhancers are genetic control elements that act, through docking with host transcription factors, as a decisive regulatory unit for efficient initiation of the productive virus cycle. Animal models are required for studying the function of enhancers paradigmatically in host organs. Here, we have sought to quantitatively assess the establishment, maintenance, and level of in vivo growth of enhancerless mutants of murine cytomegalovirus in comparison with those of an enhancer-bearing counterpart in models of the immunocompromised or immunologically immature host. Evidence is presented showing that enhancerless viruses are capable of forming restricted foci of infection but fail to grow exponentially.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-06-01 |