6533b7d1fe1ef96bd125d91a

RESEARCH PRODUCT

Comparison of high-pressure liquid chromatography (HPLC) and Griess reagent-spectroscopic methods for the measurement of nitrate in serum from healthy individuals in the Nordic countries

Pål RustadDag O. AndersenTine Lise LarsenMohammad Azam MansoorGeorge W. FrancisValentina Nilsen

subject

Adultinorganic chemicalsAdolescentClinical BiochemistryHigh-performance liquid chromatographyYoung Adultchemistry.chemical_compoundNitrateGriess testSulfanilamidesHumansHplc methodReference standardsChromatography High Pressure LiquidAgedAged 80 and overNitratesChromatographySpectrum Analysisorganic chemicalsfood and beveragesGeneral MedicineMiddle AgedReference StandardsEthylenediaminesSerum samplesBioavailabilitychemistryHealthy individuals

description

Bioavailability of NO can be estimated by measuring the concentration of nitrate (NO(3)) in serum. However, the methods used for the measurement NO(3) in plasma or serum show a great degree of variation. Therefore, we compared two analytical methods for the measurement of NO(3) in serum.The concentration of NO(3) in 600 serum samples collected from healthy individuals was determined by the HPLC and by the Griess reagent-spectroscopic method.The concentration of NO(3) in the samples was 29.4+/-16.1 micromol/L and 26.2+/-14.0 micromol/L (mean+/-SD) measured by HPLC and Griess reagent-spectroscopic method respectively (p0.0001). We detected a significant correlation between the two methods (R=0.81, p0.0001).A significant correlation between the two methods may suggest that either method can be used for the measurement of NO(3) in serum, however the Griess reagent-spectroscopic method measures lower concentrations of NO(3) than the HPLC method.

yearjournalcountryeditionlanguage
2008-12-01Clinical Biochemistry
https://doi.org/10.1016/j.clinbiochem.2008.08.083