Laimas slimības ierosinātāja Borrelia burgdorferi ārējās virsmas proteīna BB0663 iegūšana un struktūras analīze

6533b7d2fe1ef96bd125db46

RESEARCH PRODUCT

Laimas slimības ierosinātāja Borrelia burgdorferi ārējās virsmas proteīna BB0663 iegūšana un struktūras analīze

Sergejs Koļesovs

subject

Borrelia burgdorferi lipoproteīni ārējas virsmas proteīni Laimas slimība Bioloģija proteīnu kristalizācija

description

Darba mērķis bija iegūt Laimas slimības izraisītāja Borrelia burgdorferi (B. burgdorgeri) celma B31 ārējās virsmas proteīna BB0663 struktūru un veikt šīs struktūras analīzi, lai iegūtu plašākas zināšanas par baktērijas patoģenēzes mehānismiem. Darba rezultātā BB0663 proteīns tika uzproducēts Escherichia coli šūnās, attīrīts un kristalizēts. Ar rengenstruktūranalīzes palīdzību tika iegūti šī kristāla difrakcijas dati. Izmantojot iegūtos datus, tika noteikta BB0663 proteīna 3D struktūra. Struktūras analīze parādīja, ka B. burgdorferi BB0663 proteīns ir līdzīgs baktērijas Novosphingobium aromaticivorans (N. aromaticivorans) proteīnam, kuram ir noteikta struktūra, bet nav zināma funkcija. Pētījumi veikti Latvijas Biomedicīnas pētījumu un studiju centrā Dr. biol. Kalvja Branguļa vadībā.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/48258

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