6533b7d2fe1ef96bd125df22

RESEARCH PRODUCT

Fast Isolation by AFLP of Sequences Containing Repeats

Kaisa Rikalainen

subject

medicine.medical_specialtyAmplified Fragment Length Polymorphism AnalysisComputational biologyBiologygenomic DNAchemistry.chemical_compoundNucleic acid thermodynamicschemistryMolecular geneticsmedicineDirect repeatMicrosatelliteAmplified fragment length polymorphismDNA

description

Fast isolation by AFLP of sequences containing repeats (FIASCO) is a rapid and simple method for separating microsatellite-containing DNA fragments from genomic DNA de novo. The method takes the advantage of the amplified fragment length polymorphism (AFLP) technique that relies on effective digestion-ligation reaction. The repeat-containing fragments are selectively hybridized to biotinylated probes and harvested by streptavidin-coated magnetic beads. The enriched microsatellite-containing fragments can be cloned and sequenced to yield a variety of microsatellite loci for applications in many different fields in molecular genetics.

yearjournalcountryeditionlanguage
2013-01-01
https://doi.org/10.1007/978-1-62703-389-3_4