6533b7d2fe1ef96bd125df49
RESEARCH PRODUCT
Cell wall-degrading enzymes produced in vitro by isolates of Phaeosphaeria nodorum differing in aggressiveness
V. DumortierF. LalaouiBernard PaulP. Halamasubject
chemistry.chemical_classificationbiologyPlant ScienceCellulaseHorticulturebiology.organism_classificationEsteraseMicrobiologyPhaeosphaeria nodorumCell wallButyrate esteraseEnzymechemistryBiochemistryGeneticsXylanasebiology.proteinPectinaseAgronomy and Crop Sciencedescription
The relationships between in vitro production of cell wall-degrading enzymes and aggressiveness of three Phaeosphaeria nodorum isolates were investigated. When grown in liquid medium containing 1% cell wall from wheat leaves as the carbon source, the isolates secreted xylanase, α-arabinosidase, β-xylosidase, polygalacturonase, β-galactosidase, cellulase, β-1,3-glucanase, β-glucosidase, acetyl esterase and butyrate esterase. Time-course experiments showed different levels of enzyme production and different kinetics between isolates. A highly aggressive isolate produced more xylanase, cellulase, polygalacturonase and butyrate esterase than did the two weakly aggressive isolates. Xylanase was the most active polymer-degrading enzyme produced, suggesting a key role during pathogenesis by P. nodorum.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-12-01 | Plant Pathology |