6533b7d2fe1ef96bd125e97e
RESEARCH PRODUCT
A New Fluorescent Method of Detection Structural and Functional Properties Of Cells and Immune State Of Organism
Meirovics ImantsKalniņa IntaKlimkāne LauraBrūvere Rūtasubject
Cell membraneMembrane probeImmune statemedicine.anatomical_structureImmune systemPolarity (international relations)ChemistryLymphocytemedicineBiophysicsLipid bilayerFluorescencedescription
It is very important for clinics to receive information about properties of immune competent cell lymphocyte (ly) with the express method. Fluorescence methods of analysis settled down these requirements [1], At Riga Technical University was synthesised a new fluorescent probe-derivative of 3-aminobenzanthrone — ABM ( conditional name). ABM is neutral membrane probe, non-toxic for cells, photostable, sensitive to polarity changes of microenvironment, localised in the depth of phospholipid bilayer of cell membrane. Flow cytometric experiments observed strong bimodal distribution with high (90%) and low (10%) ABM fluorescence intensity (F) respectively [2, J.Fluorescence (accepted)]. The spectral characteristics of ABM in cell suspension and several parameters of the immune state are determined in different pathologies.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 |