Effect of acyl-CoA oxidase activity on the accumulation of gamma-decalactone by the yeast Yarrowia lipolytica: a factorial approach.

6533b7d2fe1ef96bd125ec1f

RESEARCH PRODUCT

Effect of acyl-CoA oxidase activity on the accumulation of gamma-decalactone by the yeast Yarrowia lipolytica: a factorial approach.

Yves Waché Jean-marc Nicaud Erandi Escamilla García Jean-marc Belin

subject

0106 biological sciences Yarrowia lipolytica MESH: Enzyme Activation Metabolic Clearance Rate MESH: Factor Analysis Statistical Yarrowia Biology modèle Models Biological 01 natural sciences Applied Microbiology and Biotechnology Lactones 03 medical and health sciences Enzyme activator Species Specificity MESH: Computer Simulation 010608 biotechnology Combinatorial Chemistry Techniques Acyl-CoA oxidase MESH: Species Specificity Computer Simulation 030304 developmental biology chemistry.chemical_classification MESH: Metabolic Clearance Rate 0303 health sciences Oxidase test métabolisme des acides gras Acyl-CoA oxidase activity MESH: Acyl-CoA Oxidase MESH: Models Biological Yarrowia [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology General Medicine Peroxisome biology.organism_classification Yeast Enzyme Activation Biochemistry chemistry lactone Molecular Medicine MESH: Combinatorial Chemistry Techniques MESH: Yarrowia Acyl-CoA Oxidase Factor Analysis Statistical Lactone MESH: Lactones

description

International audience; beta-Oxidation is a cyclic pathway involved in the degradation of lipids. In yeast, it occurs in peroxisomes and the first step is catalyzed by an acyl-CoA oxidase (Aoxp). The yeast Yarrowia lipolytica possesses several genes (POX) coding for Aoxps. This study is based on the factorial analysis of results obtained with the many POX derivative strains that have been constructed previously. The effect of interactions between Aoxps on the acyl-CoA oxidase (Aox) activity was important even at the second order. We then investigated the effect of Aox activity on growth and lactone production. Aox activity was correlated with acidification of the medium by cells and with cellular growth but not with lactone production, although Aox activity on short chains was inversely correlated with lactone accumulation. Due to the poor correlation between Aox activity and lactone production, the modeling of this parameter gave no satisfactory results but growth depending on Aox activity was modeled.

year	journal	country	edition	language
2007-10-01

10.1002/biot.200700085 https://hal.archives-ouvertes.fr/hal-00315501