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RESEARCH PRODUCT
Orange proteomic fingerprinting: From fruit to commercial juices.
Elisa FasoliAlfonsina D'amatoPier Giorgio RighettiErnesto F. Simó-alfonsoMaría Jesús Lerma-garcíasubject
0301 basic medicineProteomicsProteomeOrange (colour)01 natural sciencesAnalytical ChemistryBeverages03 medical and health sciencesTandem Mass SpectrometryLc ms msFood scienceOrange juiceLC-MS/MSPeptide ligandOrange fruitPlant ProteinsOrange juiceCombinatorial peptide ligand library; LC-MS/MS; Orange fruit; Orange juice; Protein; Proteomics; Food Science; Analytical ChemistryChromatographyChemistryProtein010401 analytical chemistryGeneral Medicine0104 chemical sciences030104 developmental biologyFruitProteomeCombinatorial peptide ligand libraryCitrus × sinensisFood ScienceCitrus sinensisdescription
Combinatorial peptide ligand library technology, coupled to mass spectrometry, has been applied to extensively map the proteome of orange pulp and peel and, via this fingerprinting, to detect its presence in commercial orange juices and drinks. The native and denaturing extraction protocols have captured 1109 orange proteins, as identified by LC-MS/MS. This proteomic map has been searched in an orange concentrate, from a Spanish juice manufacturer, as well as in commercial orange juices and soft drinks. The presence of numerous orange proteins in commercial juices has demonstrated the genuineness of these products, prepared by using orange fruits as original ingredients. However, the low number of identified proteins in sparkling beverages has suggested that they were prepared with scarce amounts of fruit extract, thus imparting lower quality to the final products. These findings not only increase the knowledge of the orange proteome but also present a reliable analytical method to assess quality and genuineness of commercial products.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-05-27 | Food chemistry |