6533b7d2fe1ef96bd125f214

RESEARCH PRODUCT

Effets du resvératrol et de formulations de micro-nutriments alimentaires sur les fonctions du muscle squelittique chez la souris et sur les cellules musculaires en culture

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A concept that has emerged in recent years is the use of micronutrients as therapeutic agents known for their anti-inflammatory, antioxidant, cardio-protective or anticarcinogenic proprieties. In this work, we studied the in vivo and in vitro mechanism of action of resveratrol and two natural formulations, one involved in the antioxidant effects (product "X") and the other (product "Y") involved in the correction of lipid disorders in people affected by metabolic syndrome. We characterized the pro-differentiating effect of a natural polyphenol, resveratrol, on C2C12 skeletal muscle cell line. This study showed that this polyphenol can induce the expression of muscle differentiation factors (myogenin, Scrp3) and increases the level of myosin, a protein involved in muscular contraction. Finally, resveratrol can modulate the expression of microRNA, such as miR-133b involved in the muscular differentiation process. Concerning oxidative stress, the results shows that "X" is capable both, to slightly modulate expression of anti-radicalar defense genes in gastrocnemius muscle (SIRT1, SOD1, UCP2, NRF1) but also, in C2C12 murine skeletal muscle cells (PRDX1, NRF2, PGC1α). The results in the gastrocnemius showed a rise of the overall radicalar defense potential by using KRL assay. A decrease of intracellular ROS was observed with the DHE probe in C2C12 cells. The study of "Y" has shown that it is able to slightly modulate the expression of genes involved in energetic metabolism (Gs1, Srebp1c, Fabp3, VLCAD, Pparβ) in C2C12 cells. Our results also shown that "Y" induces an anti-inflammatory effect by reducing the number of lymphocytes in mice fed by a High Fat/ High Sucrose diet. However, "Y" is not an agonist of PPARα and PPARγ in our conditions. The overall results obtained in this work confirms that micronutrients can induce slightly effects on gene expression. Long-term studies are needed to understand and confirm their true impact on organism