6533b7d3fe1ef96bd125fbb1

RESEARCH PRODUCT

Luminometric Sub-nanoliter Droplet-to-droplet Arrays (LUMDA-chip) for Drug Screening on Phase I Metabolism Enzymes

Giuseppe Domenico ArrabitoC GalatiS CastellanoBruno Giuseppe Pignataro

subject

Ink-Jet printing biochip biosensors array

description

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. themost relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

yearjournalcountryeditionlanguage
2013-01-01
10.1039/c2lc40948hhttp://hdl.handle.net/10447/74599