6533b7d3fe1ef96bd12600ee
RESEARCH PRODUCT
Activity of laccase on unbleached and bleached thermomechanical pulp
Liisa ViikariStina GrönqvistAnna SuurnäkkiJohanna BuchertK. Rantanensubject
RadicalBioengineeringTrametes hirsutaengineering.materialApplied Microbiology and BiotechnologyBiochemistryPeroxidelaw.inventionColloidchemistry.chemical_compoundstomatognathic systemlawOrganic chemistryElectron paramagnetic resonanceLaccaseActivity of laccase on pulpbiologyChemistryPulp (paper)LaccaseActivation of fibresbiology.organism_classificationstomatognathic diseasesengineeringBiotechnologyMyceliophthora thermophilaNuclear chemistrydescription
The introduction of value-added properties to pulp fibres is an attractive proposition. One interesting option is targeted modification of fibre surfaces by enzymatic activation. In this work, the activity of laccase on pulp and pulp fractions from thermomechanical pulp (TMP) and peroxide bleached TMP was studied on the basis of consumption of the co-substrate oxygen in the reaction and by studying the formation of radicals in the pulp material as analysed by electron paramagnetic resonance spectroscopy (EPR). Laccases obtained from Trametes hirsuta and Myceliophthora thermophila were used in the study. Laccases were found to be active on pulp material of unbleached TMP, whereas only fines from bleached TMP reacted in laccase treatment. Dissolved and colloidal substances (DCS) were assumed to have a mediating role in the laccase-catalysed oxidation of the fibre-bound material.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-03-01 |