6533b7d3fe1ef96bd12601e8

RESEARCH PRODUCT

Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium

V. La BellaR. GuarneriS ScondottoGiovanni SavettieriGiuseppe SalemiFederico PiccoliDonatella Ferraro

subject

Central nervous systemFlunitrazepamBiologySettore BIO/19 - Microbiologia GeneraleBiochemistrygamma-Aminobutyric acidGABACellular and Molecular NeurosciencemedicineAnimalsBinding siteCells Culturedgamma-Aminobutyric AcidNeuronsFetusCell DifferentiationGeneral MedicineHormonesIn vitroCulture MediaCell biologymedicine.anatomical_structureneuronal cultureCell cultureCerebral cortexSettore MED/26 - NeurologiaFlunitrazepamNeurosciencemedicine.drug

description

[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.

yearjournalcountryeditionlanguage
1990-08-01Neurochemical Research
https://doi.org/10.1007/bf00968553