Resveratrol, a chemopreventive agent, disrupts the cell cycle control of human SW480 colorectal tumor cells

6533b7d3fe1ef96bd1260273

RESEARCH PRODUCT

Resveratrol, a chemopreventive agent, disrupts the cell cycle control of human SW480 colorectal tumor cells

Patricia Passilly-degrace Mustapha Cherkaoui Malki Norbert Latruffe Brigitte Jannin Dominique Delmas

subject

DNA Replication endocrine system diseases Cell Cyclin A Adenocarcinoma Cyclin B Protein Serine-Threonine Kinases Resveratrol S Phase chemistry.chemical_compound CDC2 Protein Kinase Stilbenes CDC2-CDC28 Kinases Tumor Cells Cultured Genetics medicine Anticarcinogenic Agents Humans Cyclin B1 Phosphorylation skin and connective tissue diseases Cyclin Cyclin-dependent kinase 1 biology Kinase Cell growth organic chemicals Cell Cycle Cyclin-Dependent Kinase 2 Cyclin-dependent kinase 2 food and beverages General Medicine Cell cycle Flow Cytometry Cyclin-Dependent Kinases Growth Inhibitors Neoplasm Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure Biochemistry chemistry Resveratrol Enzyme Induction biology.protein Cancer research Colorectal Neoplasms Protein Processing Post-Translational Cell Division

description

Resveratrol is a natural polyphenolic compound produced by a number of plants and found in high amount in peanuts, seeds, grapes or berries as source of human nutrition. Epidemiological studies strongly suggest that resveratrol may act as a cancer chemopreventive compound. The mechanism by which resveratrol inhibits cell proliferation was studied in human colorectal tumor SW480 cell line. The results show that resveratrol strongly inhibits cell proliferation at the micromolar range in a time- and dose-dependent manner. Resveratrol appears to block the cell cycle at the transition --> G2/M since inhibition of [(3)H]-thymidine incorporation is not observed, while there is an increase of the cell number in S phase. During this inhibition process, resveratrol increases the content of cyclins A and B1 as well as cyclin-dependent kinases Cdk1 and Cdk2. Moreover, resveratrol promotes Cdk1 phosphorylation. In conclusion, resveratrol exerts a strong inhibition of SW480 human colorectal tumor cell proliferation at least by modulating cyclin and cyclin-dependent kinase activities.

year	journal	country	edition	language
2002-07-18	International Journal of Molecular Medicine

https://doi.org/10.3892/ijmm.10.2.193