6533b7d3fe1ef96bd1260b81

RESEARCH PRODUCT

Expression of properdin in human monocytes

Johannes MöstChristine ClausH. W. Löms Ziegler-heitbrockManfred P. DierichHartwig P. HuemerM StrobelWilhelm SchwaebleK. B. M. Reid

subject

AdultLipopolysaccharidesLipopolysaccharidemedicine.medical_treatmentMolecular Sequence DataEnzyme-Linked Immunosorbent AssayBiologyurologic and male genital diseasesBiochemistryMonocytesCell LineInterferon-gammachemistry.chemical_compoundTumor Cells CulturedmedicineHumansRNA MessengerNorthern blotBase SequenceProperdinTumor Necrosis Factor-alphaMacrophagesMonocyteBlotting NorthernMolecular biologyRecombinant ProteinsComplement systemCytokinemedicine.anatomical_structurechemistryCell cultureImmunologyAlternative complement pathwayCytokinesTetradecanoylphorbol AcetateProperdinInterleukin-1

description

Properdin is the only known positive regulator of the alternative pathway of complement activation. Northern blot analysis of cell lines derived from fibroblasts, B-cells, hepatoma cells, and cells of the monocyte-macrophage lineage revealed properdin expression only in the myelomonocytic cell line HL-60, in the monoblastic cell line U-937 and in the monocytic line Mono Mac 6. Culture of Mono Mac 6 cells for 24 h with phorbol 12-myristate 13-acetate, bacterial lipopolysaccharide and the cytokines interleukin-1 beta and tumour necrosis factor-alpha enhanced mRNA abundance, with the strongest effect (tenfold) being observed with the lipopolysaccharide. In contrast, recombinant interferon-gamma consistently halved properdin mRNA abundance. The same pattern was found for the secretion of properdin as detected by ELISA of Mono Mac 6 supernatants. The suppressive effect of interferon-gamma on properdin mRNA abundance was also demonstrated for primary blood monocytes. The data suggest that the expression and secretion of this complement regulatory protein by monocytes is differentially regulated by cytokines and link the immune response with alternative pathway activation.

yearjournalcountryeditionlanguage
1994-02-01European Journal of Biochemistry
https://doi.org/10.1111/j.1432-1033.1994.tb18555.x