6533b7d3fe1ef96bd1260baa
RESEARCH PRODUCT
Protein-membrane interaction probed by single plasmonic nanoparticles.
Andreas JanshoffCristina L BaciuJan BeckerCarsten Sönnichsensubject
StreptavidinMaterials scienceNanoparticleMolecular Probe TechniquesBioengineeringNanotechnologyResonance (particle physics)Spectral lineQuantitative Biology::Subcellular Processeschemistry.chemical_compoundProtein Interaction MappingGeneral Materials ScienceSurface plasmon resonanceSpectroscopyLipid bilayerPlasmonPlasmonic nanoparticlesbusiness.industryChemistryMechanical EngineeringCell MembraneMembrane ProteinsBiological membraneGeneral ChemistrySurface Plasmon ResonanceCondensed Matter PhysicsDark field microscopyMembraneTransmission electron microscopyBiotinylationParticleOptoelectronicsNanoparticlesbusinessdescription
We present a nanosized and addressable sensor platform based on membrane coated plasmonic particles and show unequivocally the covering with lipid bilayers as well as the subsequent detection of streptavidin binding to biotinylated lipids. The binding is detected on membrane covered gold nanorods by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel. Our approach allows for local analysis of protein interaction with biological membranes as a function of the lateral composition of phase separated membranes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-05-08 | Nano letters |