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RESEARCH PRODUCT

The Use of Atomic Force Microscopy in Determining the Stiffness and Adhesion Force of Human Dentin After Exposure to Bleaching Agents

María PalomaresLeopoldo FornerManuel Salmerón-sánchezJosé AmengualCarmen Llena

subject

Materials scienceDentistryCarbamide PeroxideMicroscopy Atomic Forcechemistry.chemical_compoundstomatognathic systemHardnessElastic ModulusMaterials TestingImage Processing Computer-AssistedTooth BleachingmedicineDentinHumansUreaAdhesion forceHydrogen peroxideGeneral DentistryAtomic force microscopybusiness.industryStiffnessHydrogen PeroxideNanoindentationOxidantsPeroxidesDrug Combinationsmedicine.anatomical_structurechemistryIntertubular dentinDentinStress Mechanicalsense organsCarbamide peroxidemedicine.symptombusinessAlgorithmsBiomedical engineering

description

Introduction: Oxidant bleaching agents may induce several alterations on mineralized teeth tissues. Our aim is to study,at the ultrastructurallevel,mechanicalmodifications induced on dentin after exposure to different bleaching agents.Methods: Nanoindentation performed with atomic force microscopy was used to measure changes in dentin stiffness as well as the adhesion force between the tip and the tissue both in intertubular and peritubulardentin.Foreachspecimen,dentinlocalization, and bleaching agent, 100 independent nanoindentations were performed. Carbamide peroxide (30%) and hydrogenperoxide(35%) wereusedasbleaching agents. Results: A significant reduction of both stiffness and adhesion force was found for both carbamide and hydrogen peroxide in peritubular and intertubular dentin. Conclusions:The use of bleaching agents led to a significant reductionin dentinlocal (at the nanoscale) mechanical properties. (J Endod 2009;35:1384‐1386)

yearjournalcountryeditionlanguage
2009-10-01Journal of Endodontics
https://doi.org/10.1016/j.joen.2009.06.023