Ganoderma lucidum 9621 ārpusšūnu ligninolītisko enzīmu aktivitātes noteikšana

6533b7d3fe1ef96bd1260e74

RESEARCH PRODUCT

Ganoderma lucidum 9621 ārpusšūnu ligninolītisko enzīmu aktivitātes noteikšana

subject

lakāze peroksidāzes Ganoderma lucidum ligninolītiskie enzīmi Bioloģija lignīna degradācija

description

Signe Vadone (2019): Ganoderma lucidum 9621 ārpusšūnu ligninolītisko enzīmu aktivitātes noteikšana. Darbā noteikta un salīdzināta G. lucidum 9621 lakāzes un peroksidāžu aktivitāte četru dažādu piedevu šķidrajās barotnēs – ½ MEB(+ Mn2+; + 2% Lg; + 2% Lg + Mn2+). Veikta kvantitatīva ligninolītisko enzīmu aktivitātes noteikšana, izmantojot spektrofotometrijas metodes. Darbā tika salīdzināta dažādu glabāšanas apstākļu (+4˚C, -20˚C un liofilizācijas) ietekme uz G. lucidum 9621 ligninolītisko enzīmu aktivitāti un inkubācijas beigās noteikta sēņu micēlija biomasa. Noskaidrots, ka augstāko ārpusšūnu ligninolītisko enzīmu aktivitāti G. lucidum 9621 iegūst 6.-10. inkubācijas dienā un lielāka aktivitāte ir paraugiem, kuri inkubēti barotnēs ar lignīna piedevu. Ieteicamie apstākļi enzīmu glabāšanai tika noteikta sasaldēšana -20˚C un liofilizācija. Atslēgvārdi: Ganoderma lucidum, lignīna degradācija, ligninolītiskie enzīmi, lakāze, peroksidāzes.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/48273

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