6533b7d3fe1ef96bd1261618
RESEARCH PRODUCT
Superparamagnetic γ-Fe2O3 nanoparticles with tailored functionality for protein separation
Vadim KsenofontovMohammed Ibrahim ShukoorMuhammad Nawaz TahirWerner E.g. MüllerFilipe NatalioWolfgang TremelPatrick TheatoHeinz C. SchröderHelen Annal Theresesubject
Surface PropertiesAllosteric regulationNanoparticleLigandsFerric CompoundsCatalysisMagneticsProtein purification2'5'-Oligoadenylate SynthetaseMaterials ChemistryAnimalsParticle Sizechemistry.chemical_classificationBinding SitesMolecular StructurebiologyImmunomagnetic SeparationMetals and AlloysRNADNA-Directed RNA PolymerasesGeneral ChemistryPolymerbiology.organism_classificationPoriferaSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsSpongeEnzymeBiochemistrychemistryCeramics and CompositesNanoparticlesPeptidesSuperparamagnetismdescription
Polymer coated superparamagnetic gamma-Fe(2)O(3) nanoparticles were derivatized with a synthetic double-stranded RNA [poly(IC)], a known allosteric activator of the latent (2-5)A synthetase, to separate a single 35 kDa protein from a crude extract which cross reacted with antibodies raised against the sponge enzyme.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-11-09 | Chemical Communications |